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KMID : 0613820230330090724
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Journal of Life Science
2023 Volume.33 No. 9 p.724 ~ p.729
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Copper Regulates Apelin Expression in L6 Skeletal Muscle Cells
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Kwon Ki-Sang
Park Jin-Sol
Choi Young-Eun
Lee Eun-Ryeong
Yoo Jae-Eun
Park Hye-Won
Kwon O-Yu
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Abstract
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In this experiment, we aimed to investigate the role of copper in regulating the biosynthesis of a myokine called apelin in mammalian skeletal muscle cells. Our approach involved culturing skeletal muscle cells and subjecting them to treatments with copper sulfate or a copper chelator known as bathocuproinedisulfonic acid (BCS). We employed standard techniques, such as reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting, to assess the synthesis of apelin at different stages, including transcription, translation, and post-translational modifications. Our findings demonstrated that copper had an inhibitory effect on apelin biosynthesis at all three stages: transcription, translation, and post-translation. However, when we treated the cells with BCS, the biosynthesis of apelin was restored to its original state. This finding suggests that copper is required for the synthesis of apelin in mammalian skeletal muscle cells. This study represents the first documented evidence of the inorganic copper-dependent regulation of apelin biosynthesis, shedding light on potential strategies for the prevention and treatment of sarcopenia induced by copper imbalances.
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KEYWORD
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Apelin, bathocuproinedisulfonic acid (BCS), copper sulfate, skeletal muscle cells
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